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SpectraWiz Software General Help
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Experiment Procedures.."how to get started taking data"
ToolBar Icons..........areas on the screen for mouse operation
System Indicators......show you what's on/off at a glance
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Experiment Procedures //////////////////////////////////////////
Ensure that you have installed the calibration coefficients for
your unit. Use the Setup->Unit calibration coefficients menu
to enter the 3 coefficient values found on the spectrometer
label. This information tells the software how to provide the
wavelength readouts. For a single unit EPP2000 spectrometers,
select unit 1 with physical address 1.
If you are interested in emmission applications, ie: looking
at light sources, LEDS, laser diodes, plasma, or even fluor-
escence, then you can start observing right away in the "Scope
mode" which is the default view mode at start-up. Otherwise,
for applications requiring spectroscopy modes such as Absor-
bance or Transmission or Reflectance (same as Transmission),
selecting the proper "View mode" after taking a Dark and Ref-
erence scan is simple enough. Note the "Dark bulb" and the
"Light bulb" icons on the toolbar for this.
Taking a Dark scan for the Scope mode becomes important with
detector integration times well above 250ms (above 1/4 second).
Although it is not required, doing so will eliminate detector
structure baseline, which is a pixel non-uniformity constant.
Always take a "new dark" after changing system parameters such
as samples to averaging, smoothing, and detector integration.
To take a dark scan, you must block the light signal input to
the spectrometer or turn off the light for a moment.
There is an alternative approach to taking the Dark scan. This
makes it a bit easier because you don't have to block the input
signal. Instead of using the left mouse click on the Dark light
bulb icon, use a right click to "zero the Dark". Now, ensure
that the Temperature compensation selection in the setup menu
is turned on (enabled). This feature evaluates the dark level
in the "optical black" region of the detector and removes this
level from the input signal on an ongoing basis. This bit of
magic eliminate the need for a dark level shutter.
In all modes, X zooming and Y re-scaling allow regions of
interest to be easily viewed. Remember, X zooming allows
exclusion of areas that may cause auto Y re-scaling to fail
because of large peaks on left or right. A little "hands on"
allows simple navigation with the tool bar.
Before "viewing" Absorbance or Transmittance we must first
setup some basic system parameters and save a "dark" and "ref-
erence" scan. This is performed in the default "Scope" view
mode.
1. Setup and turn on your reference light source so that you
are now viewing a bell shape curve in Scope mode. For
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